Part:BBa_K4829000:Experience

Applications of BBa_K4829000

This modified T7 promoter has been used in all of our DNA constructs that are meant for mRNA synthesis/protein expressions through in-vitro transcription/translation. These DNA constructs are :

• BBa_K4829003
• BBa_K4829013
• BBa_K4829014
• BBa_K4829015
• BBa_K4829016
• BBa_K4829017
• BBa_K4829018

The mentioned constructs, on in-vitro transcription(IVT) using the MEGAscript™ T7 Transcription Kit, produce mRNA as specified on the respective part pages. The following is the nanodrop analysis of the IVT of the parts

• BBa_K4829018:

 Figure 2. Nanodrop run to check for mRNA after successful completion of IVT for part BBa_K4829018]]

• BBa_K4829003

 Figure 3. Nanodrop run to check for mRNA after successful completion of IVT for part BBa_K4829003

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